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  1. Home
  2. GeneralConcept

GeneralConcept

62 Articles
  • 256
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What are the common pluripotency markers?

  • 458
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What is meant by polymerase fidelity? What applications require a high-fidelity polymerase?

  • 1420
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What is nested PCR?

  • 292
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What factors are critical for multiplex PCR?

  • 528
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What are PCR artifacts?

  • 310
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What factors contribute to PCR-introduced mutations?

  • 380
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What types of mutations can be caused by PCR?

  • 548
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What is PCR overcycling? How do I know if my product is overcycled?

  • 508
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What are PCR inhibitors?

  • 518
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If no amplification products are obtained, what parameters should be considered first when troubleshooting?

  • 4995
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What is the role of magnesium in PCR, and what is the optimal concentration?

  • 4256
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Should I use a three-step or a two-step PCR protocol?

  • 411
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How should lyophilized PCR premixes be stored?

  • 346
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What precautions should be taken when using inosine-containing primers?

  • 380
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How should oligos be purified for PCR?

  • 1358
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What primer concentration should be used for PCR?

  • 5868
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How do I calculate the melting temperature (Tm) of primers?

  • 619
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What PCR product size is optimal for real-time PCR?

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