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  1. Home
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  3. qPCR

qPCR

24 Articles
  • 438
  • 0

What is the storage temperature of Premix Ex Taq™ (Probe qPCR)?

  • 447
  • 0

What is the storage temperature of TB Green® Premix Ex Taq™ (Tli RNase H Plus)?

  • 518
  • 0

What is the difference between RR390A (Premix Ex Taq™ (Probe qPCR)) and RR039A (Premix Ex Taq™ DNA Polymerase (Perfect Real Time) kits?

  • 613
  • 0

What is the role of Tli RNase H in qPCR reaction?

  • 513
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Which SYBR Premix should I choose – RR420 or RR820? What is the difference between these premixes?

  • 434
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Can you mix the ROX™ reference dyes and TB Green® Premix Ex Taq™ to help avoid pipetting errors?

  • 650
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What is the composition of TB Green® Premix Ex Taq™?

  • 406
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Why is the amplicon length so limited in qPCR?

  • 593
  • 0

What PCR product size is optimal for real-time PCR?

  • 494
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How much ROX is needed for each instrument? Why are the ROX concentrations different?

  • 682
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What is ROX used for?

  • 400
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Should I choose Probe or Dye Based chemistry for my qPCR experiments?

  • 484
  • 0

What are the differences between absolute and relative quantification?

  • 541
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I understand that there are two ways to determine the Ct value. What are they?

  • 431
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What is the sensitivity of qPCR?

  • 376
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What is the appropriate number of replicates (n)?

  • 367
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What method(s) should be used for analyzing qPCR amplification products?

  • 408
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What standard samples are recommended for preparing calibration curves?

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