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  1. Home
  2. AssayTroubleshooting

AssayTroubleshooting

53 Articles
  • 654
  • 0

What should I do if purified protein fails to elute from TALON resin?

  • 325
  • 0

Why does the column turn violet upon addition of the sample/buffer containing guanidine-HCl?

  • 560
  • 0

What should I consider when multiplexing samples?

  • 4708
  • 0

What is touchdown PCR (TD-PCR) and when would I need to use it?

  • 3137
  • 0

What is nested PCR?

  • 915
  • 0

What are PCR artifacts?

  • 523
  • 0

What factors contribute to PCR-introduced mutations?

  • 699
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What types of mutations can be caused by PCR?

  • 1054
  • 0

What is PCR overcycling? How do I know if my product is overcycled?

  • 830
  • 0

What are PCR inhibitors?

  • 668
  • 0

What can I do if the PCR generates errors?

  • 814
  • 0

How can I decontaminate if I have PCR contamination?

  • 1068
  • 0

What are some sources of PCR contamination?

  • 2415
  • 0

If PCR generates a smear after running the products on a gel, what can be done to improve the results?

  • 1489
  • 0

If there are nonspecific amplification bands, what can be done to improve specificity?

  • 807
  • 0

If no amplification products are obtained, what parameters should be considered first when troubleshooting?

  • 4190
  • 0

What is the role of salt in PCR reactions?

  • 1149
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When optimizing PCR conditions, which conditions are particularly important?

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