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  1. Home
  2. Categories
  3. PCR

PCR

50 Articles
  • 2098
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What is touchdown PCR (TD-PCR) and when would I need to use it?

  • 1148
  • 0

What is nested PCR?

  • 246
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What factors are critical for multiplex PCR?

  • 442
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What are PCR artifacts?

  • 257
  • 0

What factors contribute to PCR-introduced mutations?

  • 329
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What types of mutations can be caused by PCR?

  • 451
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What is PCR overcycling? How do I know if my product is overcycled?

  • 448
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What are PCR inhibitors?

  • 263
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What can I do if the PCR generates errors?

  • 317
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How can I decontaminate if I have PCR contamination?

  • 291
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What are some sources of PCR contamination?

  • 1063
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If PCR generates a smear after running the products on a gel, what can be done to improve the results?

  • 395
  • 0

If there are nonspecific amplification bands, what can be done to improve specificity?

  • 458
  • 0

If no amplification products are obtained, what parameters should be considered first when troubleshooting?

  • 1559
  • 0

What is the role of salt in PCR reactions?

  • 3972
  • 0

What is the role of magnesium in PCR, and what is the optimal concentration?

  • 332
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How can I optimize PCR conditions for AT-rich templates?

  • 619
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Can DMSO be added to improve amplification of GC-rich templates?

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  • Nucleic Acid Purification (Macherey Nagel)
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