Can DMSO be added to improve amplification of GC-rich templates?
We have heard from customers that improved amplification of GC-rich templates was obtained by adding DMSO to reactions using PrimeSTAR MAX DNA Polymerase or CloneAmp HiFi PCR Premix. The recommended concentration of DMSO is between 2.5% and 5%.
2560 Orchard Parkway
San Jose, CA 95110
Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.