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  1. Home
  2. Categories
  3. PCR

PCR

50 Articles
  • 411
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What are the critical factors for amplification of GC-rich templates?

  • 698
  • 0

How do I determine if a template is GC rich?

  • 356
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What are the critical factors for amplification of long genomic targets?

  • 2105
  • 0

What is the optimal amount of DNA template that should be used for PCR?

  • 701
  • 0

Which extension temperature should I use, 68°C or 72°C?

  • 4256
  • 0

Should I use a three-step or a two-step PCR protocol?

  • 819
  • 0

When optimizing PCR conditions, which conditions are particularly important?

  • 400
  • 0

Are there any special considerations when handling PCR enzymes?

  • 411
  • 0

How should lyophilized PCR premixes be stored?

  • 751
  • 0

How are PCR polymerases shipped, and how should they be stored and handled after receipt?

  • 346
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What precautions should be taken when using inosine-containing primers?

  • 380
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How should oligos be purified for PCR?

  • 1358
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What primer concentration should be used for PCR?

  • 5868
  • 0

How do I calculate the melting temperature (Tm) of primers?

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