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  1. Home
  2. Categories
  3. PCR

PCR

50 Articles
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What are the critical factors for amplification of GC-rich templates?

  • 850
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How do I determine if a template is GC rich?

  • 437
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What are the critical factors for amplification of long genomic targets?

  • 2275
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What is the optimal amount of DNA template that should be used for PCR?

  • 868
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Which extension temperature should I use, 68°C or 72°C?

  • 4766
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Should I use a three-step or a two-step PCR protocol?

  • 933
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When optimizing PCR conditions, which conditions are particularly important?

  • 477
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Are there any special considerations when handling PCR enzymes?

  • 488
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How should lyophilized PCR premixes be stored?

  • 971
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How are PCR polymerases shipped, and how should they be stored and handled after receipt?

  • 442
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What precautions should be taken when using inosine-containing primers?

  • 488
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How should oligos be purified for PCR?

  • 1774
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What primer concentration should be used for PCR?

  • 6952
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How do I calculate the melting temperature (Tm) of primers?

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