How are PCR polymerases shipped, and how should they be stored and handled after receipt?
How are PCR polymerases shipped, and how should they be stored and handled after receipt?
Takara Bio's PCR polymerases are shipped on dry ice. All enzymes shipped on dry ice will be frozen, but this freezing process is gradual and one freeze-thaw cycle will not reduce enzyme activity. Before opening a new tube of enzyme, spin it briefly to collect the contents at the bottom of the tube. Polymerases should be stored at –20°C in a non-frost-free freezer (an acceptable range is between –15°C and –23°C). Long-term storage (longer than a week) at –70°C or –80°C is not recommended. Enzymes should never be allowed to reach room temperature.