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  1. Home
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  3. qPCR

qPCR

24 Articles
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Target gene expression levels are often normalized to the expression of a “housekeeping gene” (reference gene) to correct for differences in the amount of input RNA and variations in reaction efficiency. How do I select a suitable housekeeping gene?

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How can amplification of genomic DNA in total RNA samples be avoided?

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Which is a better starting sample for preparing RT-qPCR calibration curves, RNA or cDNA?

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What are the benefits and application of two-step RT-qPCR?

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What are the benefits and application of one-step RT-qPCR?

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Comparison between Takara RT-qPCR kits.

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