Comparison between Takara RT-qPCR kits.
Comparison between Takara RT-qPCR kits.
Comparison between Takara RT-qPCR kits.
|
One Step RT-qPCR kits for Probe-Based assays |
Premix |
RT Enzyme |
PCR Enzyme |
Probe |
Dye-based |
RT reaction |
Volume |
Template |
||
|
XU0002 |
5X One Step PrimeScript™ III RT-qPCR Kit, no ROX, GPR |
200 Rxns |
5X |
PrimeScript III |
Takara Taq |
Y |
No |
52°C 5 min |
25 ul |
10 pg - 1 μg of RNA |
|
638337 |
5X One Step PrimeScript™ III RT-qPCR Kit, no ROX, GPR |
1,000 Rxns |
5X |
PrimeScript III |
Takara Taq |
Y |
No |
52°C 5 min |
25 ul |
10 pg - 1 μg of RNA |
|
XU0001 |
5X One Step PrimeScript™ III RT-qPCR Kit, ROX plus, GPR |
200 Rxns |
5X |
PrimeScript III |
Takara Taq |
Y |
No |
52°C 5 min |
25 ul |
10 pg - 1 μg of RNA |
|
638336 |
5X One Step PrimeScript™ III RT-qPCR Kit, ROX plus, GPR |
1,000 Rxns |
5X |
PrimeScript III |
Takara Taq |
Y |
No |
52°C 5 min |
25 ul |
10 pg - 1 μg of RNA |
|
RR600A |
One Step PrimeScript™ III RT-PCR Kit |
200 Rxns |
2X + ROX dyes |
PrimeScript III |
Takara Taq |
Y |
No |
52°C 5 min |
25 ul |
10 pg - 1 μg of RNA |
|
RR600B |
One Step PrimeScript™ III RT-PCR Kit |
1,000 Rxns |
2X + ROX dyes |
PrimeScript III |
Takara Taq |
Y |
No |
52°C 5 min |
25 ul |
10 pg - 1 μg of RNA |
|
RR601A |
One Step PrimeScript™ III RT-qPCR Mix, with UNG |
200 Rxns |
2X + ROX dyes |
PrimeScript III |
Takara Taq |
Y |
No |
25℃ 10 min, then 52°C 5 min |
25 ul |
10 pg - 1 μg of RNA |
|
RR064A |
One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) |
100 Rxns |
NO |
PrimeScript RT enzyme Mix I |
TaKaRa Ex Taq HS |
Y |
No |
42℃ 5 min |
50 ul |
10 pg - 100 ng of RNA |
|
RR064B |
One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) |
500 Rxns |
NO |
PrimeScript RT enzyme Mix I |
TaKaRa Ex Taq HS |
Y |
No |
42℃ 5 min |
50 ul |
10 pg - 100 ng of RNA |
|
RR650A |
PrimeDirect™ Probe RT-qPCR Mix |
200 Rxns |
2X + ROX dyes |
Proprietary |
Proprietary |
Y |
No |
60℃ 5 min |
25 ul |
biological specimens, virus solutions, RNA |
|
RR650B |
PrimeDirect™ Probe RT-qPCR Mix |
1,000 Rxns |
2X + ROX dyes |
Proprietary |
Proprietary |
Y |
No |
60℃ 5 min |
25 ul |
biological specimens, virus solutions, RNA |
|
One Step RT-qPCR kits for Dye-Based assays |
Premix |
RT Enzyme |
PCR Enzyme |
Probe |
Dye-based |
RT reaction |
Volume |
Template |
||
|
RR086A |
One-Step TB Green® PrimeScript™ RT-PCR Kit II (Perfect Real Time) |
100 Rxns |
NO |
PrimeScript RT |
TaKaRa Ex Taq HS |
No |
Y |
42℃ 5 min |
50 ul |
10 pg - 100 ng of RNA |
|
RR086B |
One-Step TB Green® PrimeScript™ RT-PCR Kit II (Perfect Real Time) |
500 Rxns |
NO |
PrimeScript RT |
TaKaRa Ex Taq HS |
No |
Y |
42℃ 5 min |
50 ul |
10 pg - 100 ng of RNA |
|
Preamplification of limited RNA samples |
||||||||||
|
638554 |
Prelude™ One-Step PreAmp Master Mix |
10 Rxns |
2X |
Proprietary |
Proprietary |
Y |
Y |
42°C 10 min |
50 ul |
100 pg mRNA or 10 ng total RNA |
|
638553 |
Prelude™ One-Step PreAmp Master Mix |
40 Rxns |
2X |
Proprietary |
Proprietary |
Y |
Y |
42°C 10 min |
50 ul |
100 pg mRNA or 10 ng total RNA |