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  3. Genome Editing

Genome Editing

63 Articles
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Which cells should I use for genome-wide sgRNA screen?

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Why do I need to use next-generation sequencing (NGS) to analyze the results of the screen? How can I perform this analysis?

  • 283
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Why do we need multiple guides targeting the same gene?

  • 298
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Why are guide RNA libraries supplied on lentivirus vectors but not on regular plasmids?

  • 360
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What are positive and negative CRISPR library screens?

  • 297
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Pooled sgRNA libraries vs. pooled RNAi libraries vs. targeted arrayed sgRNA libraries

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What are some example applications of pooled whole-genome sgRNA library screens?

  • 319
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Where can I find more information about CRISPR/Cas9-mediated gene knockout?

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How do I perform a genome-wide CRISPR knockout screen?

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