• 221
• 0

Activity-based CRISPR scanning uncovers allostery in DNA methylation maintenance machinery

• 254
• 0

Can the Guide-it SNP Screening Kit only be used to detect single-nucleotide substitutions? Would it be suitable for detecting longer insertions?

• 209
• 0

Are there any limitations or special considerations regarding what target sequences can be analyzed with the Guide-it SNP Screening Kit?

• 255
• 0

How sensitive is the Guide-it SNP Screening Kit to the presence of mutations close to the site of the nucleotide substitution being assayed?

• 237
• 0

How clean does the PCR of the genomic target sequence need to be for the Guide-it SNP Screening Kit?

• 236
• 0

Can the Guide-it SNP Screening assay be used to distinguish homozygotes from heterozygotes?

• 254
• 0

Can the protocol for the Guide-it SNP Screening Kit be adapted for use with 384-well plates?

• 300
• 0

What cell densities are suitable for analysis with the Guide-it SNP Screening Kit?

• 266
• 0

Which cell types have been analyzed using the Guide-it SNP Screening Kit? Can the kit be used for suspension cells?

• 273
• 0

Do I need to use column-purified genomic DNA for the Guide-it SNP Screening Kit?

• 256
• 0

Can the Guide-it SNP Screening Kit be used to detect successful homologous recombination (HR) in edited populations?

• 235
• 0

Can the Guide-it SNP Screening Kit be used for genotyping?

• 302
• 0

Can I use the kit if I have no idea what the substitution is?

• 267
• 0

Does the Guide-it SNP Screening Kit allow for detection of any nucleotide substitution regardless of the genomic target site?

• 241
• 0

Can a qPCR thermal cycler be used for fluorescence detection step of Guide-it SNP Screening Kit?

• 240
• 0

What do I need for the detection of the assay signal?

• 265
• 0

What is included in the Guide-it SNP Screening Kit?

• 263
• 0

How does Guide-it Flapase detect the edited nucleotide?