• 90
• 0

What is the recommended number reads when your sample tissue does not cover the entire Tile area?

• 118
• 0

How much PhiX should I spike in to my library before sequencing?

• 114
• 0

What are the PhiX recommendations for the NovaSeq X Plus?

• 88
• 0

How deep should I sequence Curio Seeker libraries?

• 88
• 0

For the Dual Indexing Primer kit, what orientation are the sequences listed in the User Guide table?

• 83
• 0

Where can I find the bam file where reads are associated with bead barcodes and/or molecular barcodes?

• 96
• 0

What do I do if the pipeline says it cannot find my STAR reference when it clearly exists?

• 101
• 0

How do I fix the following error message? Remote resource not found: https://api.github.com/repos/nextflow-io/commercial_seeker/contents/main.nf

• 78
• 0

What do I do if the FORMATCONVERT step fails with the following error?

• 95
• 0

CURIOSEEKER_GEN_GENE_BARCODE_UMI_DB step failed with the following error. How do I resolve it?

• 78
• 0

If I have multiple regions or tissues of interest on a tile, how do I select only the beads for the region I am interested in?

• 101
• 0

How do you get untrimmed reads from the sequencer?

• 89
• 0

What method of clustering do we use for the UMAP?

• 80
• 0

Do you have any guidance on trimming FASTQ files to 2 x 50?

• 83
• 0

How do I recalculate "median reads/UMI" after filtering out beads not covered by tissue?

• 83
• 0

What if my library has indications of PCR-bubble formation?

• 89
• 0

What is difference between the Dual Indexing primer kit V1 and V2?

• 101
• 0

Do you need to optimize the permeabilization step for Curio Seeker?