• 1308
• 0

What is ROX used for?

• 657
• 0

Should I choose Probe or Dye Based chemistry for my qPCR experiments?

• 701
• 0

What are the differences between absolute and relative quantification?

• 870
• 0

I understand that there are two ways to determine the Ct value. What are they?

• 626
• 0

What is the appropriate number of replicates (n)?

• 669
• 0

What method(s) should be used for analyzing qPCR amplification products?

• 470
• 0

Can I use the NucleoProtect reagent for collecting nasal swabs?

• 626
• 0

What standard samples are recommended for preparing calibration curves?

• 566
• 0

Target gene expression levels are often normalized to the expression of a “housekeeping gene” (reference gene) to correct for differences in the amount of input RNA and variations in reaction efficiency. How do I select a suitable housekeeping gene?

• 557
• 0

How can amplification of genomic DNA in total RNA samples be avoided?

• 760
• 0

Which is a better starting sample for preparing RT-qPCR calibration curves, RNA or cDNA?

• 452
• 0

Do you have any special recommendations for isolating viral RNA from breast milk?

• 484
• 0

Is the RLT buffer from Qiagen compatible with NucleoMag Virus? Customer is isolating Covid-19 virus from various samples stored in the RLT buffer.

• 713
• 0

Can I store samples in the RA1 lysis buffer?

• 403
• 0

Can the NucleoSpin Gel & PCR Clean-up kit be used for removing the TB Green Dye?

• 536
• 0

Is it OK to freeze cells in the MR1+TCEP at -80 C? I couldn't find freezing recommendations in the user manual for the NucleoMag RNA kit.

• 460
• 0

Customer’s stool samples were collected in ithe Genotek Omnigen collection tubes (OMR-200). What are the recommendations for Genotek Omnigen and NucleoSpin® DNA Stool kit?

• 352
• 0

Can I use a software other than Cogent NGS Analysis Pipeline for the analysis of the sequencing reads generated by the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?