Takara Bio Support Portal

Search FAQs

basic

advanced

Exact phrase:

  • Home
  • Categories
    Nucleic Acid Purification (Macherey Nagel)
    NGS
    PCR
    In-Fusion
    qPCR
    -- All Categories --
  • Sign in
  • Sign up
  • Home
  • Categories
    Nucleic Acid Purification (Macherey Nagel)
    NGS
    PCR
    In-Fusion
    qPCR
    -- All Categories --
  • |
  • Submit new ticket
  • Sign in
  • Sign up
  1. Home
  2. ProdProtocol

ProdProtocol

443 Articles
  • 513
  • 0

Do I need to use column-purified genomic DNA for the Guide-it SNP Screening Kit?

  • 497
  • 0

Can a qPCR thermal cycler be used for fluorescence detection step of Guide-it SNP Screening Kit?

  • 478
  • 0

What do I need for the detection of the assay signal?

  • 585
  • 0

What oligos do I need to order to perform the assay?

  • 493
  • 0

What is the workflow for the Guide-it SNP Screening Kit?

  • 497
  • 0

How can I increase the frequencies of edited clones with only one modified allele and the other one encoding the wild-type sequence?

  • 545
  • 0

What are additional methods for improving HDR efficiency?

  • 687
  • 0

How do I design my HDR template?

  • 520
  • 0

What additional kits are needed for preparing NGS libraries downstream of screening?

  • 537
  • 0

What is a typical timeline for a genome-wide CRISPR screen using a pooled lentiviral sgRNA library?

  • 917
  • 0

How much DNA do I need to isolate prior to performing NGS?

  • 447
  • 0

Why do you recommend a 30–40% transduction efficiency and not higher?

  • 529
  • 0

Why is the expression level of Cas9 important?

  • 632
  • 0

How many total transduced cells do I need for a whole genome sgRNA-library screen and why?

  • 504
  • 0

Why do I need to use next-generation sequencing (NGS) to analyze the results of the screen? How can I perform this analysis?

  • 571
  • 0

What are positive and negative CRISPR library screens?

  • 543
  • 0

What should I do if a portion of the recombinant protein passes through a TALON CellThru column without binding to the column?

  • 389
  • 0

Why does the eluted protein/wash solution look pink?

    • first
    • prev
    • Page 7 of 25
    • next
    • last

Popular Knowledgebase Articles

  • Customer’s stool samples were collected in ithe Genotek Omnigen collection tubes (OMR-200). What are the recommendations for Genotek Omnigen and NucleoSpin® DNA Stool kit?
    • 975
    • 1
  • What is the diameter of the NucleoMag Magnetic bead particles? 
    • 857
    • 0
  • Are NucleoMag Beads Silica-based?
    • 791
    • 0
  • What are the dimensions of the NucleoVac™ 96 Vacuum Manifold?
    • 700
    • 0

Company Sites

Home

New products

Special offers

Instruments & reagent services

Contact Us

Technical support

Customer service

Sales

Shipping & delivery

Categories

  • Nucleic Acid Purification (Macherey Nagel)
  • NGS
  • PCR
  • In-Fusion
  • qPCR
  • -- All Categories --

Connect With Us

  • Takara Bio USA support portal
  • Biotechnology reagent provider
  • 2560 Orchard Parkway
    San Jose, CA 95110


    Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

  • 800.662.2566
  • [email protected]
  • https://www.takarabio.com/
    Powered by LiveHelpNow customer support suite