• 112
• 0

We do not have internet access on our Linux server. When executing Seeker v3.0 pipeline with Singularity, the pipeline is unable to download containers for subread, SAMtools, and STAR. This usually results in NextFlow throwing a “Join mismatch” type error. How do I solve this?

• 56
• 0

Once I generate my Seeker pipeline output, what can I do next to analyze my data?

• 79
• 0

Where can I find the BAM file where reads are associated with bead barcodes and/or molecular barcodes?

• 68
• 0

Why is the FORMATCLEANUP step running out of memory and failing with an error?

• 59
• 0

How do I decrease or increase the UMI threshold used to remove bead barcodes?

• 93
• 0

I am encountering the following error when executing the Seeker Primary Analysis Pipeline. What’s wrong?

• 66
• 0

Why do cell barcodes in my Trekker data have low overlap with cell barcodes from the single-nucleus sequencing data?

• 170
• 0

Where can I find the bam file where reads are associated with bead barcodes and/or molecular barcodes?

• 178
• 0

What do I do if the pipeline says it cannot find my STAR reference when it clearly exists?

• 235
• 0

How do I fix the following error message? Remote resource not found: https://api.github.com/repos/nextflow-io/commercial_seeker/contents/main.nf

• 162
• 0

What do I do if the FORMATCONVERT step fails with the following error?

• 194
• 0

CURIOSEEKER_GEN_GENE_BARCODE_UMI_DB step failed with the following error. How do I resolve it?

• 174
• 0

What if my library has indications of PCR-bubble formation?

• 307
• 0

What are additional methods for improving HDR efficiency?

• 377
• 0

Should I be worried about false positives or off-target effects when screening using the Guide-it CRISPR Genome-Wide sgRNA Library System?

• 344
• 0

What should I do if a portion of the recombinant protein passes through a TALON CellThru column without binding to the column?

• 234
• 0

Why does the eluted protein/wash solution look pink?

• 294
• 0

Why does the column turn brown?