• 206
• 0

Can I use In-Fusion Cloning to assemble a covalently linked linear DNA molecule?

• 236
• 0

Is In-Fusion Cloning compatible with vectors carrying repeated sequences?

• 244
• 0

Are large cloning vectors compatible with In-Fusion Cloning?

• 258
• 0

Do I have to dephosphorylate the termini of a linearized vector for In-Fusion Cloning?

• 308
• 0

Does In-Fusion Cloning preserve the restriction site(s) used to linearize the vector?

• 291
• 0

What cloning vectors are compatible with In-Fusion Cloning?

• 310
• 0

What is the smallest DNA fragment compatible with In-Fusion Cloning?

• 232
• 0

What is the largest DNA fragment compatible with In-Fusion Cloning?

• 301
• 0

Do I have to purify the PCR-amplified insert and/or vector prior to performing the In-Fusion Cloning reaction?

• 331
• 0

Can I split the homologous 15-nt overlap between the insert and vector, or adjacent inserts?

• 263
• 0

Can I clone multiple fragments into one vector in a single In-Fusion Cloning reaction?

• 252
• 0

Do PCR-generated 3' A-overhangs interfere with In-Fusion Cloning?

• 234
• 0

What PCR polymerases are recommended for amplification of the In-Fusion cloning insert?

• 212
• 0

What oligonucleotide quality is required for an In-Fusion PCR primer?

• 245
• 0

Do I need to use phosphorylated PCR primers for In-Fusion Cloning?

• 320
• 0

For In-Fusion Cloning, is it a problem if the 15-bp region of homology is present more than once in the vector? Will multiple recombination products result?

• 235
• 0

Can small external sequences be included in the In-Fusion PCR primer?

• 283
• 0

How do I clone my gene of interest in the same translational reading frame as a tag present in the cloning vector (e.g., fluorescent protein, Myc, HA, etc.)?