For In-Fusion Cloning, is it a problem if the 15-bp region of homology is present more than once in the vector? Will multiple recombination products result?
For In-Fusion Cloning, is it a problem if the 15-bp region of homology is present more than once in the vector? Will multiple recombination products result?
Internal recombination events at sites other than those adjacent to the vector linearization site are extremely rare. Therefore, even if your desired region of homology is present more than once in the vector sequence, unwanted recombination events are unlikely to occur.