Do PCR-generated 3' A-overhangs interfere with In-Fusion Cloning?

related articles

• What is the difference between In-Fusion Snap Assembly and In-Fusion Snap Assembly EcoDry?
  • 157
  • 0
• What is Cloning Enhancer (CE)?
  • 112
  • 0
• Does the In-Fusion Cloning method introduce errors into the sequence?
  • 81
  • 0
• What is the difference between In-Fusion Snap Assembly and In-Fusion HD Cloning?
  • 198
  • 0
• In-Fusion HD and In-Fusion Snap Assembly bundles include different PCR polymerases. Should I expect a difference in performance?
  • 93
  • 0
• How does In-Fusion Snap Assembly compare to NEBuilder HiFi DNA Assembly?
  • 149
  • 0
• What are the requirements for a homologous overlap that will facilitate a successful In-Fusion Cloning reaction?
  • 73
  • 0
• How do I generate homologous overlaps between the termini of cloning inserts and linearized vectors?
  • 107
  • 0
• How do I design PCR primers carrying 15-nt overhangs complementary to the termini of the linearized vector or adjacent insert?
  • 102
  • 0
• What is the optimal length of the homologous overlap between the termini of the PCR-amplified insert and linearized cloning vector?
  • 68
  • 0