• 254
• 0

What oligos do I need to order to perform the assay?

• 239
• 0

What is the workflow for the Guide-it SNP Screening Kit?

• 237
• 0

How can I increase the frequencies of edited clones with only one modified allele and the other one encoding the wild-type sequence?

• 246
• 0

What are additional methods for improving HDR efficiency?

• 267
• 0

What are the effects of chromatin configuration on HDR efficiency?

• 218
• 0

Does the HDR insertion site need to be directly next to the PAM?

• 319
• 0

Is it helpful to use HDR templates that introduce silent mutations in the PAM sequence or sgRNA seeding region?

• 485
• 0

What are optimal lengths for HDR template homology arms?

• 248
• 0

With ssODN or ssDNA, is it advantageous to use HDR templates that are homologous to the sense or antisense strand at the genomic target region?

• 398
• 0

How do ssODNs differ from ssDNA? When is it better to use one vs. the other?

• 262
• 0

What are the advantages and disadvantages of using single-stranded vs. double-stranded HDR templates for engineering gene knockins?

• 320
• 0

How do I design my HDR template?

• 224
• 0

What is HDR for Gene Editing?

• 283
• 0

What additional kits are needed for preparing NGS libraries downstream of screening?

• 311
• 0

What is the advantage of having an mCherry reporter gene in the sgRNA vector?

• 272
• 0

Where can I find all the vector maps for the Guide-it CRISPR Genome-Wide sgRNA Library System?

• 262
• 0

What are the advantages of your lentiviral packaging system, and how is it different from other libraries?

• 257
• 0

Does every sgRNA in the library work?