Takara Bio Support Portal

Search FAQs

basic

advanced

Exact phrase:

  • Home
  • Categories
    Nucleic Acid Purification (Macherey Nagel)
    NGS
    PCR
    In-Fusion
    qPCR
    -- All Categories --
  • Sign in
  • Sign up
  • Home
  • Categories
    Nucleic Acid Purification (Macherey Nagel)
    NGS
    PCR
    In-Fusion
    qPCR
    -- All Categories --
  • |
  • Submit new ticket
  • Sign in
  • Sign up
  1. Home
  2. Categories
  3. Genome Editing

Genome Editing

63 Articles
  • 643
  • 0

Why is guide RNA scaffold design an important feature of the library?

  • 530
  • 0

What is the Brunello algorithm that was used to create the library?

  • 441
  • 0

Why are there four sgRNAs per gene and not eight?

  • 436
  • 0

Why are there 76,612 different guides in the Guide-it CRISPR Genome-Wide sgRNA Library?

  • 379
  • 0

Which genes are targeted using your library? Where can I find a list of the targeted genes?

  • 461
  • 0

What is the design of the vector backbones used in your library?

  • 386
  • 0

What are the advantages of using an sgRNA library in Takara Bio’s Lenti-X Single Shots format vs. starting from plasmid or lentiviral particles?

  • 520
  • 0

The Guide-it library is supplied in a lyophilized Lenti-X Single Shots format. What does that mean?

  • 527
  • 0

What are the kit components of the Guide-it CRISPR Genome-Wide sgRNA Library System?

  • 447
  • 0

Why should I buy the Guide-it CRISPR Genome-Wide sgRNA Library System?

  • 483
  • 0

What is a typical timeline for a genome-wide CRISPR screen using a pooled lentiviral sgRNA library?

  • 379
  • 0

Is there any screening data for the Guide-it CRISPR Genome-Wide sgRNA Library System?

  • 491
  • 0

Should I be worried about false positives or off-target effects when screening using the Guide-it CRISPR Genome-Wide sgRNA Library System?

  • 740
  • 0

How much DNA do I need to isolate prior to performing NGS?

  • 441
  • 0

Are the guide RNAs evenly represented in your library? Does this change when producing and transducing virus?

  • 388
  • 0

Why do you recommend a 30–40% transduction efficiency and not higher?

  • 450
  • 0

Why is the expression level of Cas9 important?

  • 553
  • 0

How many total transduced cells do I need for a whole genome sgRNA-library screen and why?

    • first
    • prev
    • Page 3 of 4
    • next
    • last

Popular Knowledgebase Articles

  • Customer’s stool samples were collected in ithe Genotek Omnigen collection tubes (OMR-200). What are the recommendations for Genotek Omnigen and NucleoSpin® DNA Stool kit?
    • 856
    • 1
  • What is the diameter of the NucleoMag Magnetic bead particles? 
    • 752
    • 0
  • Are NucleoMag Beads Silica-based?
    • 692
    • 0
  • What are the dimensions of the NucleoVac™ 96 Vacuum Manifold?
    • 602
    • 0

Company Sites

Home

New products

Special offers

Instruments & reagent services

Contact Us

Technical support

Customer service

Sales

Shipping & delivery

Categories

  • Nucleic Acid Purification (Macherey Nagel)
  • NGS
  • PCR
  • In-Fusion
  • qPCR
  • -- All Categories --

Connect With Us

  • Takara Bio USA support portal
  • Biotechnology reagent provider
  • 2560 Orchard Parkway
    San Jose, CA 95110


    Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

  • 800.662.2566
  • [email protected]
  • https://www.takarabio.com/
    Powered by LiveHelpNow customer support suite