Why should I buy the Guide-it CRISPR Genome-Wide sgRNA Library System?
Why should I buy the Guide-it CRISPR Genome-Wide sgRNA Library System?
-sgRNAs are designed using the Brunello algorithm
High confidence that a high percentage of the individual sgRNAs are active and specifically target the expected gene. The high sgRNA activity also supports the use of a smaller number of sgRNAs/gene.
-Targets >19,000 genes
The user can perform an unbiased analysis by screening against the entire human genome.
-Includes four different guides per gene
Strike the perfect balance—high confidence that the screening data is real without the high cost and workload of lower quality libraries with more guides per gene.
-Optimized sgRNA scaffold design
The scaffold sequence of each sgRNA is designed to have a greater affinity for Cas9, resulting in a higher editing efficiency.
-The library is preamplified and aliquoted
Maintaining sgRNA representation when amplifying the library in plasmid form in preparation for a genome-wide screen is hard work. We have done those steps for you. Simply add water to two vials of the transfection mix and add the mix to your packaging cells to obtain high-titer lentivirus easily.
-sgRNA representation is confirmed in every batch by NGS
We sequenced our library after cloning, within the viral particles, and after transduction of target cells to verify that our library and protocol result in the representation of all 76,000+ sgRNAs in the starting cell population pre-screening, so you will lose no data.
-Uses the Lenti-X 4th generation packaging system
High-titer virus is essential for library screens. Lenti-X packaging leads to a consistent titer of 107–108 IFU per ml with this library, so you won't waste time optimizing lentivirus production.
-The library is supplied in a lyophilized ""Single Shots"" format
There is no need to amplify the library—each vial contains all the necessary plasmids (library and lentiviral packaging plasmids) and transfection reagents to generate self-inactivating lentiviral particles containing a complete sgRNA library targeting the entire human genome (>19,000 genes). Simply resuspend the lyophilized transfection mix in water and add the mixture to Lenti-X 293T cells.
-The library vector includes an mCherry reporter gene
It's easy to confirm transduction efficiency to ensure only a single sgRNA per cell is introduced using the mCherry reporter gene.
-Lenti-X 293T cells are included
Use the cells that we have validated for this library system. They transfect efficiently and produce high-titer virus.
-Functionally validated in an actual screen
High confidence of success. Many commercially available libraries have never been tested by the vendor in a real screen. We have tested this library and present the data on our website.
-Lenti-X GoStix Plus are included
Measure the titer of your lentiviral supernatant in just 10 minutes using a smartphone app.