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  2. AssayPrinciple

AssayPrinciple

118 Articles
  • 262
  • 0

What are additional methods for improving HDR efficiency?

  • 273
  • 0

What are the effects of chromatin configuration on HDR efficiency?

  • 227
  • 0

Does the HDR insertion site need to be directly next to the PAM?

  • 348
  • 0

Is it helpful to use HDR templates that introduce silent mutations in the PAM sequence or sgRNA seeding region?

  • 518
  • 0

What are optimal lengths for HDR template homology arms?

  • 270
  • 0

With ssODN or ssDNA, is it advantageous to use HDR templates that are homologous to the sense or antisense strand at the genomic target region?

  • 287
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What are the advantages and disadvantages of using single-stranded vs. double-stranded HDR templates for engineering gene knockins?

  • 343
  • 0

How do I design my HDR template?

  • 323
  • 0

What is the advantage of having an mCherry reporter gene in the sgRNA vector?

  • 263
  • 0

Does every sgRNA in the library work?

  • 336
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Why is guide RNA scaffold design an important feature of the library?

  • 317
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What is the Brunello algorithm that was used to create the library?

  • 241
  • 0

Why are there 76,612 different guides in the Guide-it CRISPR Genome-Wide sgRNA Library?

  • 267
  • 0

How much DNA do I need to isolate prior to performing NGS?

  • 278
  • 0

Are the guide RNAs evenly represented in your library? Does this change when producing and transducing virus?

  • 241
  • 0

Why do you recommend a 30–40% transduction efficiency and not higher?

  • 283
  • 0

Why is the expression level of Cas9 important?

  • 340
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How many total transduced cells do I need for a whole genome sgRNA-library screen and why?

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