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  2. AssayPrinciple

AssayPrinciple

117 Articles
  • 219
  • 0

Are there any special considerations that must be taken into account when performing AMPure XP purification on libraries prepared with the ThruPLEX DNA-Seq Kit?

  • 228
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How does the smaller number of UMI (144) provide a complete diversity of tagged molecules in ThruPLEX Tag-seq kits?

  • 263
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Are the PicoPLEX Gold libraries compatible with the Illumina Patterned Flow cell sequencing platforms such as Novaseq 6000?

  • 172
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What makes the PicoPLEX Single Cell WGA Kit suited for SNP genotyping?

  • 176
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Sigma-Aldrich sells a GenomePlex WGA kit. Does it contain the same technology as the PicoPLEX Single Cell WGA Kit?

  • 175
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What are the indexing options for the PicoPLEX Gold Single Cell DNA-Seq Kit?

  • 179
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Can we use barcodes designed in our laboratory for PicoPLEX Gold Single Cell DNA-Seq Kit?

  • 174
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Do we have to purchase barcoded oligonucleotides separately for PicoPLEX Gold Single Cell DNA-Seq Kit?

  • 223
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Can I use the PicoPLEX Gold Single Cell DNA-Seq Kit for whole-genome de novo sequencing from a single cell?

  • 222
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Can I use indexing primers from vendors other than Takara Bio USA?

  • 225
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What is the purpose of the DNA SMART Custom Read2 Seq Primer, include in the DNA SMART™ ChIP-Seq Kit?

  • 197
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Can the DNA SMART Poly(dA) Primer  anneal to internal /A -rich sequences in the genomic DNA?

  • 191
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Why is there no size selection and clean-up not before the PCR step within the DMA SMART ChIP-Seq protocol as in other kits?

  • 205
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How does the ChIP Elute work?

  • 221
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Can the Guide-it SNP Screening assay be used to distinguish homozygotes from heterozygotes?

  • 249
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How does Guide-it Flapase detect the edited nucleotide?

  • 224
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How can I increase the frequencies of edited clones with only one modified allele and the other one encoding the wild-type sequence?

  • 230
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What are additional methods for improving HDR efficiency?

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