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  2. ProdProtocol

ProdProtocol

443 Articles
  • 10603
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What is the role of magnesium in PCR, and what is the optimal concentration?

  • 694
  • 0

How can I optimize PCR conditions for AT-rich templates?

  • 1908
  • 0

Can DMSO be added to improve amplification of GC-rich templates?

  • 652
  • 0

What are the critical factors for amplification of GC-rich templates?

  • 614
  • 0

What are the critical factors for amplification of long genomic targets?

  • 3015
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What is the optimal amount of DNA template that should be used for PCR?

  • 1563
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Which extension temperature should I use, 68°C or 72°C?

  • 6185
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Should I use a three-step or a two-step PCR protocol?

  • 1149
  • 0

When optimizing PCR conditions, which conditions are particularly important?

  • 625
  • 0

Are there any special considerations when handling PCR enzymes?

  • 641
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How should lyophilized PCR premixes be stored?

  • 619
  • 0

What precautions should be taken when using inosine-containing primers?

  • 728
  • 0

How should oligos be purified for PCR?

  • 4035
  • 0

What primer concentration should be used for PCR?

  • 738
  • 0

Can you mix the ROX™ reference dyes and TB Green® Premix Ex Taq™ to help avoid pipetting errors?

  • 647
  • 0

Why is the amplicon length so limited in qPCR?

  • 810
  • 0

What PCR product size is optimal for real-time PCR?

  • 800
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How much ROX is needed for each instrument? Why are the ROX concentrations different?

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