• 1554
• 0

What are the recommended insert-to-vector molar ratios for In-Fusion Cloning?

• 570
• 0

Can I use In-Fusion Cloning to clone GC-rich DNA fragments?

• 599
• 0

Can I use In-Fusion Cloning for mutagenesis?

• 480
• 0

Will In-Fusion technology allow cloning of an insert if the sites of complementarity are located at a distance from the linearized vector termini?

• 510
• 0

Do I have to dephosphorylate the termini of a linearized vector for In-Fusion Cloning?

• 616
• 0

Does In-Fusion Cloning preserve the restriction site(s) used to linearize the vector?

• 785
• 0

What cloning vectors are compatible with In-Fusion Cloning?

• 576
• 0

What is the smallest DNA fragment compatible with In-Fusion Cloning?

• 571
• 0

Do I have to purify the PCR-amplified insert and/or vector prior to performing the In-Fusion Cloning reaction?

• 675
• 0

Can I split the homologous 15-nt overlap between the insert and vector, or adjacent inserts?

• 526
• 0

Do PCR-generated 3' A-overhangs interfere with In-Fusion Cloning?

• 484
• 0

What PCR polymerases are recommended for amplification of the In-Fusion cloning insert?

• 441
• 0

What oligonucleotide quality is required for an In-Fusion PCR primer?

• 509
• 0

Do I need to use phosphorylated PCR primers for In-Fusion Cloning?

• 458
• 0

Can small external sequences be included in the In-Fusion PCR primer?

• 580
• 0

How do I clone my gene of interest in the same translational reading frame as a tag present in the cloning vector (e.g., fluorescent protein, Myc, HA, etc.)?

• 770
• 0

How can I alter the reading frame when performing In-Fusion Cloning?

• 593
• 0

How do I calculate the 15-nt overlap if the vector is linearized via restriction digest, generating a 5' or 3' overhang?