• 551
• 0

Target gene expression levels are often normalized to the expression of a “housekeeping gene” (reference gene) to correct for differences in the amount of input RNA and variations in reaction efficiency. How do I select a suitable housekeeping gene?

• 528
• 0

How can amplification of genomic DNA in total RNA samples be avoided?

• 493
• 0

What are the benefits and application of two-step RT-qPCR?

• 578
• 0

What are the benefits and application of one-step RT-qPCR?

• 510
• 0

Can I purchase RCU buffer from 740910 NucleoSpin RNA Clean-up Maxi?

• 416
• 0

What is Marcherey Nagel Carrier RNA made of?

• 411
• 0

What are the sizes of holes of the NucleoBond Smart Rack?

• 464
• 0

What are the differences between 744950 (KingFisher 96 Accessory Kit A) and 744951 (KingFisher 96 Accessory Kit)?

• 477
• 0

Can samples lysed in TriZol be used on the NucleoSpin® RNA Set for NucleoZOL™?

• 433
• 0

What are the dimensions of the NucleoSpin® 96 Plasmid Filter Plates?

• 485
• 0

What are the dimensions of the NucleoVac™ 96 Vacuum Manifold?

• 504
• 0

Can the NucleoSpin Plasmid Purification columns substitute the NucleoSpin Plasmid Transfection- grade columns to isolate Endo-free plasmid DNA?

• 354
• 0

What is the number of maximal theoretical UMI combinations in the sequencing reads generated by the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

• 375
• 0

What is the nature of Unique Molecular Identifiers (UMI) incoporated into the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico libraries?

• 339
• 0

Can I use a software other than Cogent NGS Analysis Pipeline for the analysis of the sequencing reads generated by the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

• 335
• 0

What software should I use for the analysis of the sequencing reads generated by the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

• 583
• 0

What is the advantage of incoporation of Unique Molecular Identifiers (UMI)?

• 320
• 0

What is the advantage of using Unique Dual Indexes ?