• 128
• 0

Is the Seeker kit compatible with Element Biosciences’ AVITI sequencer?

• 8
• 0

For the Dual Indexing Primer kit, what orientation are the sequences listed in the User Guide table?

• 5
• 0

How do I recalculate sequencing depth after filtering out beads that are not covered by tissue?

• 6
• 0

How do I crop out a region of interest from the spatial embedding of my Seeker data?

• 8
• 0

How do I normalize my data using log-transformed count per million (CPM)?

• 6
• 0

What normalization method was used by the Seeker Primary Analysis Pipeline to generate the UMAP and clustering results in the HTML report?

• 3
• 0

What tool was used to generate the UMAP by the Seeker Primary Analysis Pipeline, and how many principal components were used?

• 8
• 0

What tool and algorithm were used for clustering by the Seeker Primary Analysis Pipeline?

• 6
• 0

What are the default parameters (e.g., the number of principal components used and the resolution for finding clusters) to reproduce the plots in the HTML report from the Seeker Primary Analysis Pipeline?

• 11
• 0

How do I trim my FASTQ pairs to be at least 50 bp?

• 9
• 0

What are the format requirements for the input FASTQ pairs?

• 8
• 0

In Read 2, are there any negatives to incorporating reads beyond the minimum 50 bp, besides needing more computing time (i.e., taking an 'as much as possible' approach)?

• 8
• 0

What versions of packages are used in the latest Seeker Primary Analysis Pipeline?

• 8
• 0

What is the structure of read 1 and read 2 in the Seeker data?

• 18
• 0

What's the pore size of Capturem membrane?

• 29
• 0

How do I obtain additional resources for protocols and bioinformatics analyses?

• 32
• 0

What thickness of tissue section is required for Trekker?

• 26
• 0

How big is the Trekker tile?