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  2. ProdDescription

ProdDescription

414 Articles
  • 548
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Should I be worried about false positives or off-target effects when screening using the Guide-it CRISPR Genome-Wide sgRNA Library System?

  • 847
  • 0

How much DNA do I need to isolate prior to performing NGS?

  • 487
  • 0

Are the guide RNAs evenly represented in your library? Does this change when producing and transducing virus?

  • 608
  • 0

How many total transduced cells do I need for a whole genome sgRNA-library screen and why?

  • 512
  • 0

Which cells should I use for genome-wide sgRNA screen?

  • 482
  • 0

Why do I need to use next-generation sequencing (NGS) to analyze the results of the screen? How can I perform this analysis?

  • 425
  • 0

Why do we need multiple guides targeting the same gene?

  • 476
  • 0

Why are guide RNA libraries supplied on lentivirus vectors but not on regular plasmids?

  • 541
  • 0

What are positive and negative CRISPR library screens?

  • 489
  • 0

Pooled sgRNA libraries vs. pooled RNAi libraries vs. targeted arrayed sgRNA libraries

  • 455
  • 0

Where can I find more information about CRISPR/Cas9-mediated gene knockout?

  • 496
  • 0

How do I perform a genome-wide CRISPR knockout screen?

  • 456
  • 0

Why does the column turn brown?

  • 516
  • 0

What is xTractor Buffer?

  • 377
  • 0

Can I elute my his-tagged protein from TALON resin with a histidine gradient?

  • 431
  • 0

Which reagents are compatible with TALON resin?

  • 404
  • 0

When can the resin no longer be used?

  • 363
  • 0

If the HisTALON Gravity Column provides 1 ml of space for the resin slurry, how much extra room is there for the sample?

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