Can I elute my his-tagged protein from TALON resin with a histidine gradient?
Can I elute my his-tagged protein from TALON resin with a histidine gradient?
Histidine is not recommended for protein elution from TALON resin because it will bind strongly to cobalt ions and cause metal ion transfer, resulting in stripping of the column. We recommend imidazole because it lacks the additional primary amine of histidine, so it binds less strongly to the metal ions and does not cause stripping of the column.