• 328
• 0

How can I alter the reading frame when performing In-Fusion Cloning?

• 243
• 0

How do I calculate the 15-nt overlap if the vector is linearized via restriction digest, generating a 5' or 3' overhang?

• 226
• 0

Why are homologous overlaps important for In-Fusion Cloning reactions?

• 267
• 0

What tools are available to assist in the design of PCR primers compatible with In-Fusion Cloning?

• 240
• 0

What is the optimal length of the homologous overlap between the termini of the PCR-amplified insert and linearized cloning vector?

• 331
• 0

How do I design PCR primers carrying 15-nt overhangs complementary to the termini of the linearized vector or adjacent insert?

• 297
• 0

How do I generate homologous overlaps between the termini of cloning inserts and linearized vectors?

• 229
• 0

What are the requirements for a homologous overlap that will facilitate a successful In-Fusion Cloning reaction?

• 392
• 0

How does In-Fusion Snap Assembly compare to NEBuilder HiFi DNA Assembly?

• 292
• 0

In-Fusion HD and In-Fusion Snap Assembly bundles include different PCR polymerases. Should I expect a difference in performance?

• 741
• 0

What is the difference between In-Fusion Snap Assembly and In-Fusion HD Cloning?

• 289
• 0

Does the In-Fusion Cloning method introduce errors into the sequence?

• 357
• 0

What is Cloning Enhancer (CE)?

• 424
• 0

What is the difference between In-Fusion Snap Assembly and In-Fusion Snap Assembly EcoDry?