What is Cloning Enhancer (CE)?
What is Cloning Enhancer (CE)?
Cloning Enhancer, or CE, is a proprietary enzyme mix for removing background plasmid DNA and PCR residue, thus eliminating the need for additional purification of PCR-amplified DNA prior to the In?Fusion reaction (see details in the In?Fusion Snap Assembly User Manual). CE is available as a separate item.
Use of CE is only appropriate if PCR amplification generates a single PCR fragment of the expected size, without a background smear. CE is a convenient tool for high-throughput applications that employ highly optimized PCR cycling conditions and primers that generate clean DNA fragments of the expected size.
The addition of CE to the In?Fusion reaction mix is not required, nor does it increase cloning efficiency—it simply replaces standard purification steps, provided that PCR yields high-quality results.