What are the requirements for a homologous overlap that will facilitate a successful In-Fusion Cloning reaction?
What are the requirements for a homologous overlap that will facilitate a successful In-Fusion Cloning reaction?
Homologous overlaps are necessary for In?Fusion Cloning. Appropriate homology consists of a 15-nt DNA sequence complementary to the 5' end of a linearized cloning vector or cloning insert. For multiple-insert cloning, we recommend increasing homology to 20 nt. Figures 2 and 3 in the In?Fusion Snap Assembly User Manual or In?Fusion Snap Assembly EcoDry User Manual provide detailed examples.