Does the In-Fusion Cloning method introduce errors into the sequence?
Does the In-Fusion Cloning method introduce errors into the sequence?
We have not seen any base slippage, base addition, or base deletion with the In?Fusion Cloning enzyme. We have cloned and sequenced over 4,000 separate clones and various human open reading frames subsequent to In?Fusion Cloning, and have rarely seen any evidence of errors at the cloning junctions (<2%). Most of the sequence errors that we have come across are clearly due to errors in primer synthesis (that is, they appear in all or many of the clones containing a particular insert). In?Fusion Cloning is ideal for making error-free fusion constructs.