• 734
• 0

Why do I have to use different magnetic devices for SPRI bead purification of cDNA?

• 541
• 0

Why do I have to perform a negative control during SMARTer cDNA synthesis?

• 542
• 0

Why should I perform a positive-control cDNA synthesis reaction?

• 489
• 0

Why do library concentrations obtained with a qPCR-based method differ from those obtained by other methods?

• 525
• 0

Why is quantification of NGS libraries by qPCR better than using other methods?

• 606
• 0

Why should I quantify my libraries prior to sequencing?

• 786
• 0

How should sequencing libraries be prepared using SMARTer stranded kits?

• 577
• 0

Do I have to scale down the Nextera XT DNA Sample Preparation Kit protocol when using 100–150 pg of ds cDNA?" "

• 571
• 0

Can I use more than 150 pg of ds cDNA for the Nextera XT DNA Sample Preparation Kit?

• 564
• 0

How do I pool cDNA libraries generated with the Low Input Library Prep kits for Illumina sequencing?

• 584
• 0

What is the expected size range of Covaris-sheared cDNA after library preparation?

• 615
• 0

How should I set up the Peak Incident Power (W) for the Covaris S220 system?

• 562
• 0

What type of Covaris machine did you use to optimize the shearing parameters?

• 606
• 0

What is the expected size range of Covaris-sheared double-stranded (ds) cDNA?

• 613
• 0

What method should I use to prepare cDNA generated with SMARTer Ultra low kits for sequencing?

• 586
• 0

How should sequencing libraries be prepared from cDNA generated with SMARTer Ultra low kits?

• 677
• 0

Can I analyze unpurified double-stranded (ds) cDNA for PCR cycle optimization?

• 602
• 0

How do I determine the double-stranded (ds) cDNA yield