What type of Covaris machine did you use to optimize the shearing parameters?
What type of Covaris machine did you use to optimize the shearing parameters?
Covaris shearing parameters, provided in the user manuals of the SMARTer Ultra low kits, were optimized using a Covaris S220 Focused-ultrasonicator.
If you are using another type of Covaris apparatus, please consult the manufacturers for the recommended parameters to ensure DNA is in the size range of 100–500 bp with a peak at approximately 200 bp.