Why should I quantify my libraries prior to sequencing?
Why should I quantify my libraries prior to sequencing?
To obtain the highest quality NGS data, loading the flow cell with an appropriate amount of library DNA is essential. An insufficient amount of library DNA will generate low-density clusters and reduced sequencing yield. Excessive amounts of library DNA may increase cluster density, resulting in poor data quality. In addition, for multiplexed sequencing, there must be an equal amount of each library in a pool to obtain a uniform number of reads across libraries. For libraries prepared for Illumina sequencing, we recommend the Library Quantification Kit.