• 333
• 0

Can I use 5X First-Strand Buffer supplied with the SMARTScribe™ Reverse Transcriptase as a substitute for the 5X Ultra Low First-Strand Buffer supplied with theSMART-Seq® mRNA Kit ?

• 349
• 0

How many reactions can I perform using different size formats of the SMART-Seq® mRNA Kit ?

• 459
• 0

Why do I need to perform Positive and Negative controls?

• 349
• 0

Can I use a carrier or co-precipitant during purification of RNA intended for the SMART-Seq mRNA cDNA synthesis?

• 342
• 0

What can I do when input RNA exhibiting a low 260/230 OD ratio?

• 310
• 0

Can I freeze collected cells prior to the SMART-Seq cDNA synthesis?

• 316
• 0

Can I use more than 1,000 cells as input for direct cDNA synthesis using SMART-Seq® mRNA kits?

• 243
• 0

Do I need to use a PhiX DNA spike-in during the sequencing SMART-Seq Stranded cDNA libraries?

• 399
• 0

Can I use a pooling option for the SMART-Seq Stranded cDNAs, generated from bulk RNA inputs?"

• 613
• 0

What RNA fragmentation method is used in the SMART-Seq Stranded workflow?

• 463
• 0

What RNA purification kits are recommended for compatibility with the SMART-Seq Stranded cDNA synthesis?

• 366
• 0

Do I have to remove rRNA prior to the SMART-Seq® Stranded cDNA synthesis?

• 568
• 0

How to transfer iPS cells to DEF-CS

• 446
• 0

What is the freeze/thaw stability of the DEF-CS 500 Basal Media and Additives (can they undergo multiple freeze thaw cycles)?

• 406
• 0

Can DEF-CS cultured iPS cells be differentiated to all 3 germ layer cells?

• 472
• 0

Can cells grown in DEF-CS be differentiated into neurons?

• 384
• 0

Can the hiPS-derived hepatocytes be dissociated and frozen?

• 492
• 0

Can the hiPS cell-derived hepatocytes be dissociated and reseeded?