Can I use 5X First-Strand Buffer supplied with the SMARTScribe™ Reverse Transcriptase as a substitute for the 5X Ultra Low First-Strand Buffer supplied with theSMART-Seq® mRNA Kit ?
Can I use 5X First-Strand Buffer supplied with the SMARTScribe™ Reverse Transcriptase as a substitute for the 5X Ultra Low First-Strand Buffer supplied with theSMART-Seq® mRNA Kit ?
No, 5X Ultra Low First-Strand Buffer supplied with the SMART-Seq® mRNAKit includes also DTT and dNTPs, that are not included n the 5X First-Strand Buffer supplied with the SMARTScribe™ Reverse Transcriptase.