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  2. ProdProtocol

ProdProtocol

443 Articles
  • 471
  • 0

Why does the column turn brown?

  • 654
  • 0

What should I do if purified protein fails to elute from TALON resin?

  • 373
  • 0

Is it necessary to add lysozyme if xTractor Buffer is used with mammalian cells?

  • 492
  • 0

Should a pH- or imidazole-based elution be used?

  • 341
  • 0

What is the maximum imidazole concentration that can be used with IMAC resins such as TALON resin?

  • 413
  • 0

Can nonphosphate-based buffer systems such as Tris or acetate buffers be used with TALON resin?

  • 512
  • 0

Can TALON columns/resin be regenerated?

  • 486
  • 0

What should be done if the protein contains a tag in the middle of the sequence, i.e., not a terminal tag?

  • 386
  • 0

What should be done if a his-tagged protein is secreted from the cell into the cell culture medium?

  • 439
  • 0

How do the purification conditions used with TALON resin maximize biological activity more effectively than nickel-based resins?

  • 655
  • 0

For indexes in 96-well-plate format: can I generate just a few samples at a time?

  • 606
  • 0

What guidelines should I follow when pooling indexes?

  • 560
  • 0

What should I consider when multiplexing samples?

  • 648
  • 0

How can I mitigate index crosstalk or ""sample bleeding"" issues?

  • 493
  • 0

What are the candidates for substitute pluripotency tests?

  • 528
  • 0

What are the substitute convenient tests for pluripotency?

  • 401
  • 0

Do you have any data/experience with using DEF-CS media on Matrigel?

  • 535
  • 0

Tips for single cell cloning.

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