How do the purification conditions used with TALON resin maximize biological activity more effectively than nickel-based resins?
How do the purification conditions used with TALON resin maximize biological activity more effectively than nickel-based resins?
Since his-tagged proteins bind more specifically to TALON resin than to nickel-based resins, one can use less stringent conditions for washing and elution, obtaining highly pure, natively folded protein with elution conditions that are closer to physiological. Also, since the metal chelator is tetradentate, there is very little leaching of the metal into the eluate.