How can I mitigate index crosstalk or ""sample bleeding"" issues?
How can I mitigate index crosstalk or ""sample bleeding"" issues?
The following steps may help you mitigate these issues:
*Use dual-indexing kits, rather than single-indexing kits, in library preparation
*Increase the percentage of PhiX spike-in, which will reduce the number of adjacent clusters with indexes
*Lower the amount of library loaded to decrease the cluster density