• 140
• 0

What is the recommended number of reads when your sample tissue does not cover the entire tile area?

• 142
• 0

How much PhiX should I spike in to my library before sequencing?

• 127
• 0

How deep should I sequence Seeker libraries?

• 14
• 0

For the Dual Indexing Primer kit, what orientation are the sequences listed in the User Guide table?

• 12
• 0

What tool and algorithm were used for clustering by the Seeker Primary Analysis Pipeline?

• 9
• 0

What are the default parameters (e.g., the number of principal components used and the resolution for finding clusters) to reproduce the plots in the HTML report from the Seeker Primary Analysis Pipeline?

• 13
• 0

How do I get untrimmed reads from the sequencer?

• 16
• 0

What are the format requirements for the input FASTQ pairs?

• 14
• 0

In Read 2, are there any negatives to incorporating reads beyond the minimum 50 bp, besides needing more computing time (i.e., taking an 'as much as possible' approach)?

• 14
• 0

What versions of packages are used in the latest Seeker Primary Analysis Pipeline?

• 15
• 0

What is the structure of read 1 and read 2 in the Seeker data?

• 17
• 0

What is the unit of the spatial coordinates from the Seeker Primary Analysis Pipeline? How do I convert them to µm?

• 21
• 0

What's the pore size of Capturem membrane?

• 39
• 0

What thickness of tissue section is required for Trekker?

• 28
• 0

How big is the Trekker tile?

• 103
• 0

What are the dimensions of 740488?

• 102
• 0

What is the conversion of coordinate system to um?

• 123
• 0

How thick should my tissue sections be for the Curio Seeker tile?