What is the structure of read 1 and read 2 in the Seeker data?

• As shown in the figure below, both molecular and cellular barcodes are in Read 1. mRNA information is in Read 2.  

Seeker 3x3 library structure. 

After proper demultiplexing, Read 1 starts with BB-1.  

Lengths for each component in R1 FASTQ: 

BB-1: 8 bp 

Linker: 18bp 

BB-2: 6 bp 

UMI: 9 bp 

Poly(dT): all remaining base pairs 

BB-1+BB-2 is the complete bead barcode that should be matched to our spatial whitelist.