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  3. his-TALON

his-TALON

32 Articles
  • 302
  • 0

What should I do if a portion of the recombinant protein passes through a TALON CellThru column without binding to the column?

  • 192
  • 0

Why does the eluted protein/wash solution look pink?

  • 224
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Why does the column turn brown?

  • 233
  • 0

What should I do if purified protein fails to elute from TALON resin?

  • 155
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What proteins have been extracted with xTractor Buffer at Takara Bio, and from what types of cells?

  • 245
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What is the effect on protein yield if xTractor Buffer is used with a fresh bacterial pellet compared to a frozen cell pellet?

  • 198
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What are the components of xTractor Buffer?

  • 194
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Is it necessary to add lysozyme if xTractor Buffer is used with mammalian cells?

  • 297
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What is xTractor Buffer?

  • 190
  • 0

Can arginine be present in buffers used with TALON resin?

  • 263
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Should a pH- or imidazole-based elution be used?

  • 217
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Can I elute my his-tagged protein from TALON resin with a histidine gradient?

  • 173
  • 0

What is the maximum PMSF concentration that can be used with TALON resin?

  • 180
  • 0

What is the maximum imidazole concentration that can be used with IMAC resins such as TALON resin?

  • 202
  • 0

Can nonphosphate-based buffer systems such as Tris or acetate buffers be used with TALON resin?

  • 248
  • 0

Which reagents are compatible with TALON resin?

  • 196
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When can the resin no longer be used?

  • 208
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Can TALON columns/resin be regenerated?

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