Can arginine be present in buffers used with TALON resin?
Can arginine be present in buffers used with TALON resin?
Arginine may be used in buffers intended for use with the TALON resin only if the final concentration is lower than 1 mM. At higher concentrations (and if using an elevated pH for adsorption) it would likely interfere with the binding of the his-tagged protein. If using an arginine-containing buffer, the pH should be maintained at ~7.2 and the sample diluted if the final arginine concentration is found to be higher than 1 mM.