What proteins have been extracted with xTractor Buffer at Takara Bio, and from what types of cells?
What proteins have been extracted with xTractor Buffer at Takara Bio, and from what types of cells?
We have tested xTractor Buffer on various E. coli host strains expressing the following proteins: 6x(HN)-LacZ (beta-galactosidase), 6x(His)-LacZ, 6x(HN)-GFPuv (Green Fluorescent Protein), 6x(His)-GFPuv, HAT-DHFR (DiHydro Folate Reductase), and 6x(HN)-Luciferase. Both beta-galactosidase and luciferase were functionally tested following purification and were found to retain their enzymatic activity after the extraction.