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  1. Home
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  3. RNA-seq general

RNA-seq general

64 Articles
  • 480
  • 0

What is the expected size distribution of double-stranded cDNA generated by SMARTer cDNA synthesis kits?

  • 553
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How do I analyze double-stranded (ds) cDNA quality?

  • 422
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Can I collect cells directly in Reaction Buffer?

  • 434
  • 0

Can I freeze collected cells prior to cDNA synthesis?

  • 448
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What is the recommended volume of Reaction Buffer for various amounts of cells when using SMARTer Ultra Low kits?

  • 433
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How do I lyse cells for direct cDNA synthesis?

  • 442
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What media have been tested for compatibility with direct cDNA synthesis from intact mammalian cells?

  • 378
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Can I use more than 1,000 cells as input for direct cDNA synthesis using SMARTer Ultra low kits?

  • 380
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How many cells can I use for direct cDNA synthesis with SMARTer Ultra low kits?

  • 396
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Which SMARTer Ultra low kit is recommended for direct cDNA synthesis from whole mammalian cells?

  • 478
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What are general considerations for cDNA synthesis directly from cells?

  • 412
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How do I shear rRNA-depleted RNA?

  • 414
  • 0

What ribosomal RNA depletion methods do you recommend?

  • 370
  • 0

Why do I need to remove ribosomal RNA?

  • 400
  • 0

Which RNA purification kits are compatible with the SMARTer Universal kit?

  • 557
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SMARTer Ultra low kits: what RNA purification kits are recommended?

  • 442
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What methods can I use to assess RNA quality and quantity?

  • 420
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What are the requirements for RNA quality and quantity when using SMARTer Ultra Low kits?

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