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  1. Home
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  3. RNA-seq general

RNA-seq general

64 Articles
  • 256
  • 0

What are the most common artifacts of cDNA synthesis with SMARTer kits?

  • 215
  • 0

Why is quantification of NGS libraries by qPCR better than using other methods?

  • 334
  • 0

What is the expected size range of fragmented, ds cDNA after library preparation with Nextera kits?

  • 224
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Do I have to scale down the Nextera XT DNA Sample Preparation Kit protocol when using 100–150 pg of ds cDNA?

  • 194
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Can I use more than 150 pg of ds cDNA for the Nextera XT DNA Sample Preparation Kit?

  • 261
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How do you explain the presence of introns in the final SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian cDNA library?

  • 312
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What are the nucleotide sequences of the adapters required for trimming prior to the software analysis of the sequencing reads?

  • 225
  • 0

Will collected cells change the volume of the cell collection buffer?

  • 255
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How should cells be collected for cDNA synthesis?

  • 260
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What are abnormal Bioanalyzer traces for SMARTer stranded kits?

  • 250
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What are abnormal Bioanalyzer traces for SMARTer Ultra low kits?

  • 388
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What are abnormal Bioanalyzer traces for all SMARTer kits?

  • 253
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Can I substitute alternative products for any of the recommended additional materials?

  • 423
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How can I ensure efficient cDNA purification using SPRI beads?

  • 262
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Why do I have to use different magnetic devices for SPRI bead purification of cDNA?

  • 205
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Why do I have to perform a negative control during SMARTer cDNA synthesis?

  • 251
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Why should I perform a positive-control cDNA synthesis reaction?

  • 220
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Why do library concentrations obtained with a qPCR-based method differ from those obtained by other methods?

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