SMARTer Ultra low kits: what RNA purification kits are recommended?
SMARTer Ultra low kits: what RNA purification kits are recommended?
Choose the most suitable RNA purification kit for your starting material (e.g., plant, tissue, mammalian cells). The RNA purification kit should be compatible with downstream cDNA synthesis and sequencing, the use of a carrier is not recommended.Note: Some plant species have high levels of polysaccharides, which may be retained in the final RNA prep. Excess polysaccharides may block the primer from binding to the RNA template, interfering with reverse transcription.
For RNA isolation from up to 1 x 105 cultured cells you may consider the NucleoSpin RNA XS kit (Cat. # 740902.10). NucleoSpin RNA XS kit specifications are provided in Table I of the NucleoSpin RNA XS Total RNA Isolation User Manual.
The use of a poly(A) carrier during RNA purification is not recommended since it may interfere with downstream oligo(dT)-primed cDNA synthesis.
If your RNA sample is dilute or was pre-purified using organic compounds, you may concentrate and clean up the RNA without the addition of a carrier using the NucleoSpin RNA Clean-up XS kit (Cat. # 740903.10) as described in the NucleoSpin RNA Clean-up XS User Manual.
Traces of organic compounds (e.g., TRIzol, ethanol) in the RNA prep may interfere with reverse transcription.