What methods can I use to assess RNA quality and quantity?
What methods can I use to assess RNA quality and quantity?
To determine the RIN and RNA quantity we suggest using the Agilent RNA 6000 Pico Kit (Agilent, Cat. # 5067-1513). If total RNA is not limiting, you may use the Agilent RNA 6000 Nano Kit (Agilent, Cat. # 5067-1511).
In our hands, the Agilent RNA 6000 Pico Kit was more accurate for assessing RNA quantity at lower concentrations compared to other assays tested.